Application of aqueous two phase systems to the recovery of bioparticulates

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University of Birmingham , Birmingham
Statementby Simon Glyn Walker.
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Open LibraryOL18096291M

Aqueous biphasic systems (ABS) or aqueous two-phase systems (ATPS) are clean alternatives for traditional organic-water solvent extraction systems. ABS are formed when either two polymers, one polymer and one kosmotropic salt, or two salts (one chaotropic salt and the other a kosmotropic salt) are mixed at appropriate concentrations or at a particular temperature.

Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membranes, viruses, enzymes, nucleic acids and other biomolecules both in industry and academia.

Although, the partition behavior involved in the method is complex and difficult to by: A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations.

Separation of biological molecules and particles in these aqueous two-phase systems. Application of aqueous two phase systems to the recovery of bioparticulates. (Thesis) Walker SG. Publisher: University of Birmingham [] Metadata Source: The British Library Type: Thesis. Abstract. No abstract supplied.

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Description Application of aqueous two phase systems to the recovery of bioparticulates PDF

About Europe PMC Cited by: 3. The phase with the highest recovery for the PEG Sodium phosphate and PEG Potassium phosphate systems was the top phase (% ± ) and bottom phase ( ± ), respectively.

These results were significantly different from the other two systems (p Cited by: 1. The major advantages of employing aqueous two-phase systems for the recovery of these studied products are highlighted, in addition to the multiple applications and variant modes that can be exploited with this extraction technique.

Application of Aqueous Two-Phase Systems for the Recovery of Bioactive Low-Molecular Weight Compounds. Separation Science and Technology: Vol. 49, No. 12, pp. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight.

In recent year, aqueous two‑phase system (ATPS) has become a proven tool used in separation and purification tech‑ nology. The application of ATPSs in clarification, partitioning and partial purification of biomolecules and bioproducts had showed the rapid development.

This method is able to give high recovery yield and high purity in a. Aqueous Two-Phase System. InMartinus Willem Beijerinck accidently found the aqueous two-phase system (ATPS) while mixing an aqueous solution of starch and gelatin.

Since then, the ATPS has been used for a range of purposes [22]. Generally, different forces, such as gravitational, flotation, partition, and frictional, act on phase.

Application of aqueous two phase systems to the recovery of bioparticulates. Author: Walker, Simon Glyn. ISNI: Awarding Body: University of Birmingham Current Institution: University of Birmingham Date of Award: Availability of Full Text.

Aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG ) and sodium citrate (SC) has been proposed to recover the valuable soluble proteins from tannery wastewater.

A sequential optimization strategy which included fractional factorial design (fFD) and central composite design (CCD) was employed to enhance the recovery. Liquid–liquid extraction (LLE), also known as solvent extraction and partitioning, is a method to separate compounds or metal complexes, based on their relative solubilities in two different immiscible liquids, usually water (polar) and an organic solvent (non-polar).

There is a net transfer of one or more species from one liquid into another liquid phase, generally from aqueous to organic. The development of recovery processes using aqueous two‐phase systems (ATPS) has been limited by the extensive experimental work required to establish the optimal system parameters to ensure selective partitioning of the product of interest.

Aqueous two-phase system (ATPS) is a liquid–liquid separation technique that has shown great potential for the extraction, recovery, and purification of a great variety of biological compounds.

Yield recovery from top phase (R T) and from bottom phase (RB) are calculated in order to evaluate the purification ratio (PR) from aqueous two phase system, according to following equations: 1 1 T V R RK = + (5) 1 B V R R K = + (6) Recovery of target protein Recovery of total protein.

Over the last years, aqueous two-phase systems (ATPS) regained an increasing interest due to their potential in the downstream processing of biomolecules. After many years with only a few articles published, a lot of effort and work has been put into studying these systems for the partitioning of a range of compounds including proteins, organic low-molecular weight molecules or metal ions.

Book January aqueous two-phase systems for the recovery of these studied products are highlighted, in addition to the multiple applications and variant modes that can be exploited with.

Aqueous two phase system (ATPS) is a liquid – liquid extraction method which employs two aqueous phases having applications in the field of biotechnology for the separation and purification of biological materials such as proteins, enzymes, nucleic acids, virus, antibodies and cell organelles.

This review discusses the basic principles of ATPS, the factors affecting partitioning. became turbid and a two-phase system was formed. The composition of this mixture was noted and provided a point on the binodal curve.

Then, M potassium phosphate buffer (pH ) was added drop-wise to the tube to get a clear one-phase system and more salt solution was added again to afford a two-phase system. Practical application of aqueous two-phase systems for the development of a prototype process for C-phycocyanin recovery from Spirulina maxima.

J Chem Technol Biotechnol.

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; – doi: /jctb Romay C, Gonzalez R. Phycocyanin is an antioxidant protector of human erythrocytes against lysis by peroxyl radicals. Aqueous Two‐Phase Systems (ATPS) is a primary recovery technique that has shown great potential for the efficient extraction and purification of high value biological compounds.

The main advantages of this technique include scaling up feasibility. An aqueous two-phase system (ATPS) based on PEG and sodium citrate was investigated for the recovery of phenolic compounds that could act as natural antioxidants for applications including cosmetics, pharmaceuticals, food additives and other industrial applications.

Aqueous two-phase systems (ATPSs) have been recognized for their applications in extraction, separation, purification, and enrichment of (bio)molecules and cells. Recently, their unique ability to create aqueous–aqueous interfaces through phase separation and the characteristics of these interfaces have created new opportunities in biomedical.

Title:The Application of Aqueous Two-phase System in the Extraction of Natural Products from Chinese Herbal Medicine: A Review VOLUME: 23 ISSUE: 6 Author(s):Cheng Xiang, Jie Chang*, Ying Ying Yue, Ju Wang and Yan Fu Affiliation:Key Laboratory of Heat Transfer Enhancement and Energy Conservation, Ministry of Education, School of Chemistry and Chemical Engineering, South China.

Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells.

Aqueous two-phase systems Aqueous two-phase systems Hatti-Kaul, Rajni Biphasic systems formed by mixing of two polymers or a polymer and a salt in water can be used for separation of cells, membranes, viruses, proteins, nucleic acids, and other biomolecules.

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The partitioning between the two phases is dependent on the surface properties and conformation of the. Development and evaluation of environmentally benign aqueous two phase systems for the recovery of bio-molecules Estimation of Scalable Video adaptation parameters for Media Aware Network Elements A review of the occurrence of pharmaceuticals and personal care products in Indian water bodies, Ecotoxicology and Environmental Safety.

The practical application of aqueous two-phase systems (ATPS) to process development has been exploited for several years for the recovery of biological products.

Unfortunately, this has not resulted in an extensive presence of the technique in commercial processes. A ‘Heat treatment aqueous two phase system’ was employed for the first time to purify serine protease from kesinai (Streblus asper) leaves.

In this study, introduction of heat treatment procedure in serine protease purification was investigated. In addition, the effects of different molecular weights of polyethylene glycol (PEGand ) at concentrations of 8, 16 and 21% (w/w. The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years.

Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water.

Similar behavior is observed when aqueous solutions of two or more incompatible polymers or.The results reported here demonstrate the potential application of ATPS to establish the conditions for an extractive fermentation prototype process for the recovery of cyanobacterial products.

AB - The potential use of aqueous two-phase systems (ATPS) to establish a viable protocol for the in situ recovery of cyanobacterial products was evaluated.Aqueous Two-Phase Systems (ATPS) have been used since their discovery for the isolation of a large number of different biological materials, such as animal and plant cells, microorganisms, viruses, cellular organelles such as chloroplasts, mitochondria, membrane vesicles, and also in the purification of proteins and nucleic acids.